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aav rg hsyn1 egfp  (Addgene inc)


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    Addgene inc aav rg hsyn1 egfp
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Aav Rg Hsyn1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+rg+hsyn1+egfp/bio_rxiv__64898__2026__03__14__711800-250-7-10?v=Addgene+inc
    Average 96 stars, based on 275 article reviews
    aav rg hsyn1 egfp - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Midbrain Tet1 dosage defines inter-individual binge-eating susceptibility"

    Article Title: Midbrain Tet1 dosage defines inter-individual binge-eating susceptibility

    Journal: bioRxiv

    doi: 10.64898/2026.03.14.711800

    (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Figure Legend Snippet: (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Techniques Used: Labeling, Two Tailed Test, Expressing, One-tailed Test



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    aav rg hsyn1 egfp  (Addgene inc)
    96
    Addgene inc aav rg hsyn1 egfp
    (A) Schematic of retrograde labeling of input projection neurons to the VTA using <t>AAV.rg-hSyn1-EGFP.</t> (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).
    Aav Rg Hsyn1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+rg+hsyn1+egfp/bio_rxiv__64898__2026__03__14__711800-250-7-10?v=Addgene+inc
    Average 96 stars, based on 1 article reviews
    aav rg hsyn1 egfp - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Journal: bioRxiv

    Article Title: Midbrain Tet1 dosage defines inter-individual binge-eating susceptibility

    doi: 10.64898/2026.03.14.711800

    Figure Lengend Snippet: (A) Schematic of retrograde labeling of input projection neurons to the VTA using AAV.rg-hSyn1-EGFP. (B) Micrograph of an exemplary coronal brain section upon machine learning-assisted brain region mapping (Aligning Big Brains & Atlases (ABBA/ImageJ)). Back-labeled neurons are highlighted in the mPFC and LHA. (C) Circos plot showing relative input strength from reward-related regions to the VTA comparing eBED-prone versus-resilient Tet1 +/– mice (upper panel); corresponding binge sizes of individual Tet1 +/- mice across 5 eBED days plotted on a Log 10 scale to visualize varied responses (lower panel; shaded area shows normal binge response). Data are presented as mean minimum to maximum values. ** P < 0.01. n = 4 mice (unpaired Student’s t -test). (D) Coronal brain schematics of mPFC subregions ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). (E) Representative micrographs of mPFC PL from eBED-prone versus-resilient Tet1 +/− mice. Scale bar 100 μm. (F) Bar graphs of raw EGFP density values from mPFC subregions hemisections ( PL : prelimbic; Cg1 : dorsal cingulate cortex; Cg2 : ventral cingulate cortex). Data are presented as mean ± SEM. * P < 0.05. n = 4 mice (two-tailed unpaired Student’s t -test). (G) Schematic illustrating chemogenetic approach to selectively inhibit VTA-projecting mPFC PL neurons using a dual AAV approach (AAV.rg-Cre + AAV-hSyn1-FLEX-hM4Di-mCherry). (H) Micrograph of VTA-projecting mPFC PL neurons expressing mCherry (red) intermingled with layer-5 pyramidal neurons (CTIP2: grey). Scale bar: 500 μm and 50 μm (insert). (I) Cumulative binge intake of hM4Di mPFC-VTA mice and controls per individual days (left panel). Data are presented as mean ± SEM. *** P < 0.001, **** P < 0.0001. n = 4–5 mice (multiple unpaired Student’s t -test); mean binge sizes of hM4Di mPFC-VTA mice and controls (right panel). Data are presented as mean and minimum to maximum. * P < 0.05, n = 4–5 mice (one-tailed unpaired Student’s t -test).

    Article Snippet: To perform brain-wide mapping of VTA inputs, AAV rg -hSyn1-EGFP (Addgene, #105553, 7E12 GC/mL) was bilaterally injected into the VTA of 6–8-week-old Tet1 +/– mice and littermate controls.

    Techniques: Labeling, Two Tailed Test, Expressing, One-tailed Test